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2 edition of Molecular characterization of the proximal human endothelial nitric oxide synthase (eNOS) promoter. found in the catalog.

Molecular characterization of the proximal human endothelial nitric oxide synthase (eNOS) promoter.

Fotula Karantzoulis-Fegaras

Molecular characterization of the proximal human endothelial nitric oxide synthase (eNOS) promoter.

by Fotula Karantzoulis-Fegaras

  • 112 Want to read
  • 19 Currently reading

Published .
Written in English


The Physical Object
Pagination274 leaves.
Number of Pages274
ID Numbers
Open LibraryOL22350297M
ISBN 100612457338

see commentary on page Endothelial nitric oxide synthase gene polymorphisms and the renal hemodynamic response to L-arginine David Z.I. Cherney1, James W. Scholey1, Joyce Zhou1, Joseph Zimpelmann2, Christopher Kennedy2, Kevin D. Burns2, Vesta Lai1 and Judith A. Miller1 1Division of Nephrology, Toronto General Hospital, University of Toronto, Toronto, Ontario, Canada and 2Division . OBJECTIVE: We previously found a T/C polymorphism in the 5'-flanking region of the endothelial nitric oxide synthase (eNOS) gene and reported that this polymorphism is strongly associated with coronary spasm. In this study, we examined whether the polymorphism is a prognostic marker in coronary spasm patients.

CoA) reductase inhibitor, on endothelial vasoactive substances using human umbilical vein endothelial cells (HUVECs). Incubation of HUVECs with fluvastatin for 12 h increased endothelial nitric oxide synthase (eNOS) mRNA expression in a concentration-dependent manner (peak, 38%, mean S.D., of the con-trol, at M fluvastatin, P ).Cited by:   Introduction. Penile erection is a hemodynamic process involving increased arterial inflow and restricted venous outflow. The role of nitric oxide (NO), generated by both endothelial NO synthase (eNOS) and neuronal NO synthase, as the main mediator of penile erection is well documented [].Nitric oxide enters smooth muscle cells and activates soluble guanylyl cyclase, leading to increased Cited by:

  In this issue of Molecular Pharmacology, Andreeva et al. (p. ) report a novel functional link between the heterotrimeric G protein Gα12 and endothelial nitric-oxide synthase (eNOS). Based on studies characterizing the interaction of Gα12 and the molecular chaperone Hsp90 and the interaction of eNOS and Hsp90, the group proposed an interaction between Gα12 and eNOS and sought to Cited by: 1.   Abstract. Objectives: To analyse the role of nitric oxide (NO) in peripheral arterial disease (PAD) and its association with inflammation and brachial artery flow-mediated dilation (BAFMD) as an estimation of endothelial al and methods: Cross-sectional study of 82 patients with ischaemia (50 with Fontaine stage II and 32 with Fontaine stage III–IV) in whom BAFMD, hsCRP and Cited by:


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Molecular characterization of the proximal human endothelial nitric oxide synthase (eNOS) promoter by Fotula Karantzoulis-Fegaras Download PDF EPUB FB2

Endothelial nitric-oxide synthase (eNOS) 1 is the enzyme responsible, in major part, for endothelial-derived NO ().Targeted inactivation of the murine eNOS locus by homologous recombination and physiologic assessment of (−/−) off-spring has reinforced the viewpoint that NO in blood vessels plays a quintessential role in regulation of local blood vessel tonus (2, 3), in remodeling of the Cited by:   Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform.

S Lamas, P A Marsden, G K Li, P Tempst, and T Michel Cardiovascular Division, Brigham and Women's Hospital, Boston, MA Cited by: Hossain M, Qadri SM, Liu L. Inhibition of nitric oxide synthesis enhances leukocyte rolling and adhesion in human microvasculature.

J Inflamm (Lond) ; doi: / [PMC free article] Cirino G, Fiorucci S, Sessa WC. Endothelial nitric oxide synthase: the Cinderella of inflammation?Cited by: References 1 Förstermann U, Closs EI, Pollock JS, Nakane M, Schwarz P, Gath I, Kleinert H.

Nitric oxide synthase isozymes: characterization, purification, molecular cloning, and functions. Hypertension. ; – Link Google Scholar; 2 Raman CS, Li H, Martasek P, Kral V, Masters BS, Poulos TL. Crystal structure of constitutive endothelial nitric oxide synthase: a paradigm for Cited by: A study demonstrated that antiserum raised against nitric oxide synthase purified from the rat cerebellum is effective in staining the enzyme in the perivascular nerves and endothelial cells.

Ca 2+ introduced from the external fluid using Ca 2+ channels of a non-L-type is a prerequisite for activating the enzyme and synthesizing NO in these.

Endothelial nitric oxide synthase (eNOS) is essential for neovascularization. Here we show that the impaired neovascularization in mice lacking eNOS is related to a defect in progenitor cell Cited by:   Nitric oxide (NO) is a key regulator of endothelial cell and vascular function.

The direct measurement of NO is challenging due to its short half-life, and as such surrogate measurements are Author: Achini K. Vidanapathirana, Benjamin J.

Pullen, Run Zhang, Run Zhang, My Ngan Duong, My Ngan Duong, J. Human endothelial nitric oxide synthase (eNOS) has been cloned and expressed inEscherichia spectroscopic properties and specific activity (– nmolmin −1 mg −1 at 37°C) of the recombinant protein are similar to those of the bovine enzyme.

FPLC and low-temperature SDS–PAGE indicate that the protein is mostly dimeric in both the absence and presence of by:   Nitric oxide (NO) is a ubiquitous intercellular messenger molecule synthesized from the amino acid L-arginine by NO synthases in diverse cells and tissues.

NO is synthesized in vascular endothelial cells and appears to play an important role in the control of blood pressure and platelet aggregation. A detailed understanding of the regulation of NO synthesis by endothelial cells has been Cited by: Nitric oxide, a key signaling molecule, is produced by a family of enzymes collectively called nitric oxide synthases (NOS).

Here, we report the crystal structure of the heme domain of endothelial. Recombinant human endothelial nitric-oxide synthase (eNOS) was prepared using a baculovirus expression system as described previously with slight modification (15, 16).

The cell pellets were suspended in buffer A (20 mM Tris-HCl, pHmM EDTA, mM EGTA, 1 mM dithiothreitol, 20 mM CHAPS, 10% glycerol, 1 μM antipain, 1 μM leupeptin, 1. The roughly 10 14 endothelial cells of our vasculature protect us against atherosclerosis and thrombosis.

A major weapon of endothelial cells to fight vascular disease is endothelial nitric oxide synthase (eNOS), an enzyme that generates the vasoprotective molecule nitric oxide (NO).Cited by:   Endothelial nitric oxide (NO) is a significant signaling molecule that regulates cerebral blood flow (CBF), playing a pivotal role in the prevention and treatment of cerebrovascular diseases.

However, achieving the expected therapeutic efficacy is difficult using direct administration of NO donors. Therefore, endothelial nitric oxide synthase (eNOS) becomes a potential therapeutic target for Cited by: Request PDF | Molecular Regulation of Inducible Nitric Oxide Synthase | This chapter summarizes the molecular mechanisms that govern inducible nitric oxide synthase (iNOS) gene expression.

Endothelial dysfunction symbolizes several pathological conditions, including altered anticoagulant and anti-inflammatory properties of the endothelium, impaired modulation of vascular growth, and dysregulation of vascular remodeling. Nevertheless, this term has been used commonly to refer to an impairment of endothelium-dependent vasorelaxation caused by a loss of nitric oxide bioactivity.

Aim: Nitric oxide (NO), produced by the polymorphic endothelial nitric oxide synthase (NOS3), plays an important role in endothelial function. The aim was to determine the effect of NOS3 polymorphisms on hypertension and cardiovascular disease (CVD) in renal allograft by: 7. Nitric oxide (NO) regulates vascular tone and local blood flow, platelet aggregation and adhesion, and leukocyte-endothelial cell interactions.

Abnormalities in NO production by the vascular endothelium result in endothelial dysfunction, which occurs in hypertension, diabetes, aging, and as a prelude to atherosclerosis. The common feature of endothelial dysfunction is a decrease in the amount Cited by: Nitric oxide stimulates migration of human endothelial and prostate cancer cells through up‐regulation of pleiotrophin expression and its receptor protein tyrosine phosphatase β/ζ we studied the possible involvement of endothelial nitric oxide synthase (eNOS) and the role of nitric oxide (NO) in the regulation of PTN expression, as well Cited by:   Endothelial nitric oxide synthase (eNOS, NOS3) is responsible for producing nitric oxide (NO)—a key molecule that can directly (or indirectly) act as a vasodilator and anti-inflammatory mediator.

In this review, we examine the structural effects of regulation of the eNOS enzyme, including post-translational modifications and subcellular Cited by:   The vascular endothelium is a monolayer of cells between the vessel lumen and the vascular smooth muscle cells.

Far from being inert, it is metabolically active and produces a variety of vasoactive mediators. Among these mediators, endothelial derived nitric oxide is essential in the maintenance of vascular homeostasis, and defects in the L-arginine: nitric oxide pathway have been Cited by:.

Background: The aim of this study was to evaluate the correlation between endothelial nitric oxide synthase (eNOS) polymorphism (−T>C) and migraine susceptibility in a meta-analysis. Methods: A literature search was performed for case–control studies from inception to J focusing on eNOS polymorphism (−T>C) and risk of migraine.

Purpose: The cascade of molecular events leading to tumor necrosis factor (TNF)-mediated tumor regression is still incompletely elucidated. We investigated the role of endothelial nitric oxide synthase in determining the tumor-selective activity of TNF. Experimental Design: Using quantitative real-time PCR, endothelial nitric oxide synthase gene levels were measured in melanoma Cited by: REDUCED EXPRESSION OF ENDOTHELIAL NITRIC OXIDE SYNTHASE IN THE LUNGS OF PATIENTS WITH PULMONARY HYPERTENSION A DEL G IAID, P H.D., AND D INA S ALEH, B.S C.

Abstract Background. Pulmonary.